Faststart taq dna polymerase roche pdf
FASTSTART TAQ DNA POLYMERASE ROCHE PDF >> READ ONLINE
VentR DNA Polymerase is a recombinant, high-fidelity thermophilic DNA polymerase with the lowest cost per reaction of any moderate-fidelity PCR polymerase. It has an error rate 5-fold lower than Taq DNA Polymerase, a characteristic derived in part from an intrinsic 3?>5? proofreading exonuclease. Taq DNA Polymerase From Thermus aquaticus BM, recombinant (E. coli) Deoxynucleoside-triphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7 5 U Trademarks FASTSTART and HIGH PURE are Trademarks of Roche. Contact and Support To ask questions, solve problems, suggest PowerQ® Taq DNA Polymerase for qPCR User Manual. Storage condition: The undiluted enzyme solution is stable when stored. Each lot of Taq DNA Polymerase is tested for activity in PCR and efficient incorporation of digoxigenin-11-dUTP, and in DNA sequencing of M13mp18ssDNA. Description: GoTaq® DNA Polymerase(a,b) is a Taq DNA polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced (a)Use of this product in the US for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. To decontaminate Taq DNA polymerase, we chose to use conditions that minimize changes in the Taq storage buffer composition in order to enable long term preservation of the enzyme at ?20°C after decontamination in batch. The tests were carried out in the storage buffer of the FastStart® Taq DNA Taq DNA Polymerase—sold under licensing arrangements with Applied Biosystems. Purchase is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process. We are CustomBiotech from Roche. Making your vision a reality. Taq 4827007103 Taq DNA Polymerase, 50 U/µl, glycerol-free solution. KU custom fill. Taq (hotstart), GMP 4659163103 FastStart Taq DNA Polymerase, GMP Grade, 5 U/µl. FastStart Taq DNA Polymerase is a chemically modified form of thermostable recombi-nant Taq DNA Polymerase that shows no activity up to 75°C. The Tth DNA Polymerase (available from Roche Applied Science) will reversely transcribe RNA templates (in the presence of Mn2+ ions) and thus FastStart Taq DNA Polymerase | Enzyme + Buffer. Catalog Number. FastStart Taq DNA Polymerase Experience Specificity Amplicon Size Specificity Sensitivity Robustness Accuracy vs. Taq Carryover Prevention up to 3 kb 1x Units/50 µl 2 Molecular Cloning yes TA clon FastStart Taq DNA Polymerase, 5 U/?l. Figure 1: Amplification of a 130-bp fragment from the plasminogen activator (tPA) gene. FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol. Taq Gold® DNA polymerase, FastStart® Taq DNA polymerase, HotStar® Taq DNA polymerase, Cheetah® Taq DNA polymerase, and Maxima Hot Start® Taq. In a reversibly-inactivated DNA polymerase, lysine residues can be reversibly blocked by chemical modification of the ?-amino group Taq Gold® DNA polymerase, FastStart® Taq DNA polymerase, HotStar® Taq DNA polymerase, Cheetah® Taq DNA polymerase, and Maxima Hot Start® Taq. In a reversibly-inactivated DNA polymerase, lysine residues can be reversibly blocked by chemical modification of the ?-amino group DNA polymerase and 18-fold lower than Taq DNA polymerase, making it the highest fidelity enzyme available (See Table I). Amplification of a 500-bp It ensures higher sensitivity, specificity, and yields compared to conventional hot start Taq DNA polymerase. It is capable of amplifying long amplicons A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2-3 times shorter. Surprising results from negative controls led to the discovery of strand-displacement ability
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