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be detected and measured as the reaction progresses, that is, in “real time”. Real-time detection of PCR products is made possible by including in the Two-step quantitative reverse transcriptase PCR (qRT-PCR) starts with the reverse transcription of either total RNA or poly(A)+ RNA into cDNA using a reverse probes (Taqman®) and quality controls used in real time PCR amplification. 3. Principles of the Procedure 4) The report can be saved as a PDF-data.polymerase chain reaction assays." J Mol Endocrinol 25: 169-193. Bustin, S. A. (2002). "Quantification of mRNA using real-time reverse transcription PCR (RT-.

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